Day 4 - How Cleaning Agents and Essential Oils Affect Our Unknown Bacteria

This experiment is to show how cleaning agents and essential oils affect our unknown bacteria. 

Materials:
Agar plate streaked with our unknown bacteria
Lysterine mouthwash
Lysol
Ginger essential oil
Lime essential oil
Clove essential oil
5 small circles of paper
Sterilized forceps
Pipet
Disposable ends for pipets


Steps:
1.  Using sterilized forceps, put 5 small pieces of paper on the agar plate: on the top, right, left, bottom, and center of the agar plate. 
2.  Draw 5 microliters of Lysterine into the pipet and expel onto one of the paper circles.
3.  Dispose of the pipet tip in the biohazard waste bag and get another sterile tip.
4.  Draw 5 microliters of Lysol into the pipet and expel onto one of the paper circles.
5.  Dispose of the pipet tip in the biohazard waste bag and get another sterile tip.
6.  Draw 5 microliters of ginger essential oil into the pipet and expel onto one of the paper circles.
7.  Dispose of the pipet tip in the biohazard waste bag and get another sterile tip.
8.  Draw 5 microliters of lime essential oil into the pipet and expel onto one of the paper circles.
9.  Dispose of the pipet tip in the biohazard waste bag and get another sterile tip.
10.  Draw 5 microliters of clove essential oil into the pipet and expel onto one of the paper circles.
11.  Dispose of the pipet tip in the biohazard waste bag.
12.  Incubate the agar plate at 35 degrees for 24 hours.
13.  Measure and record the diameters of the clear circles around the paper circles.



Results:

Our unknown bacteria

Diameters:
Lysterine mouthwash - 0 mm
Lysol - 0 mm
Ginger essential oil - 0 mm
Lime essential oil - 0 mm
Clove essential oil - 8 mm


Discussion: 
Our bacteria is slightly sensitive to clove oil.  Our bacteria is resistant to Lysterine, Lysol, ginger oil, and lime oil.